Isatins Inhibit N5-CAIR Synthetase by a Substrate Depletion Mechanism
نویسندگان
چکیده
منابع مشابه
Identification of inhibitors of N5-carboxyaminoimidazole ribonucleotide synthetase by high-throughput screening.
The increasing risk of drug-resistant bacterial infections indicates that there is a growing need for new and effective antimicrobial agents. One promising, but unexplored area in antimicrobial drug design is de novo purine biosynthesis. Recent research has shown that de novo purine biosynthesis in microbes is different from that in humans. The differences in the pathways are centered around th...
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Site-Directed Mutagenesis of Conserved Amino Acids Residues in NCarboxyaminoimidazole Ribonucleotide Mutase & Developing a High-Throughput Screen Assay for the Discovery of NCarboxyaminoimidazole Ribonucleotide Mutase Inhibitors by ZHIWEN SHI July 2014 Advisor: Dr. Steven M. Firestine Major: Pharmaceutical Sciences Degree: Master of Science The de novo purine biosynthesis pathway plays a critic...
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Acetyl coenzyme A synthetase (EC 6.2.1.1) has been examined for its ability to accept various carboxylic acids as substrates in place of acetic acid. The activity of the enzyme with these substrates was monitored using a coupled enzyme assay and high pressure liquid chromatography (HPLC) analysis. Short chain carboxylic acids were found to be active including: propionic, acrylic, fluoroacetic, ...
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Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2.1 and 2.5 A resolution, respectively. PurK belongs to the ATP grasp superfamil...
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ENZYMOLOGY AND MEDICINAL CHEMISTRY OF N-CARBOXYAMINOIMIDAZOLE RIBONUCLEOTIDE SYNTHETASE:A NOVEL ANTIBACTERIAL TARGETbyHANUMANTHARAO PARITALAAUGUST 2010Advisor: Dr. Steven M. FirestineMajor: Pharmaceutical SciencesDegree: Doctor of PhilosophyN-Carboxyaminoimidazole ribonucleotide synthetase (N-CAIRsynthetase), a key enzyme in microbial de novo purine b...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2019
ISSN: 0006-2960,1520-4995
DOI: 10.1021/acs.biochem.8b00939